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anti pan ampar
Anti Pan Ampar, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+glua2/AMPA+Receptor+(GluA2%2F3%2F4)+Antibody/pm41872515-78-48-51
Average 93 stars, based on 31 article reviews
Anti Pan Ampar, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+glua2/AMPA+Receptor+(GluA2%2F3%2F4)+Antibody/pm41872515-78-48-51
Average 93 stars, based on 31 article reviews
anti pan ampar - by Bioz Stars,
2026-10
93/100 stars
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Confocal Microscopy:Article Title: Astrocytic PYGM attenuates tau pathology by promoting lactate-mediated neuroprotection. Article Snippet: were detected using enhanced chemiluminescence (ECL) and quantified using ImageJ (NIH). .. Primary antibodies used were: anti-α-tubulin (PTM, 5442, 1:10000), anti-β-actin (Abclonal, AC026, 1:10000), anti-Caspase-3 (Cell Signaling Technology, 9662S, 1:1000), anti-Cleaved-Caspase3 (Cell Signaling Technology, 9664S, 1:1000), anti-Cleaved-PARP (Cell Signaling Technology, 9544S, 1:1000), anti-GLAST (Proteintech, 20785-1-AP, 1:1000), anti-GluA1 (Cell Signaling Technology, 13185S, 1:1000), Article Title: Astrocytic PYGM attenuates tau pathology by promoting lactate‐mediated neuroprotection Article Snippet: were detected using enhanced chemiluminescence (ECL) and quantified using ImageJ (NIH). .. Primary antibodies used were: anti‐α‐tubulin (PTM, 5442, 1:10000), anti‐β‐actin (Abclonal, AC026, 1:10000), anti‐Caspase‐3 (Cell Signaling Technology, 9662S, 1:1000), anti‐Cleaved‐Caspase‐3 (Cell Signaling Technology, 9664S, 1:1000), anti‐Cleaved‐PARP (Cell Signaling Technology, 9544S, 1:1000), anti‐GLAST (Proteintech, 20785‐1‐AP, 1:1000), anti‐GluA1 (Cell Signaling Technology, 13185S, 1:1000), Article Title: TMEM59 deficiency activates chaperone-mediated autophagy and ameliorates disease-like pathologies in tauopathy model mice. Article Snippet: Ten to twenty micrograms of protein lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting.. The blots were immunoblotted with primary antibodies, including: anti-β-actin (CST, 8457S, 1:2000), anti-ATF6 (Abcam, ab227830, 1:1000), anti-BiP (CST, C50B12, 1:1000), antiCDK5 (Santa Cruz, sc-173, 1:1000), anti-Flag (Proteintech, 20543- 1-AP, 1:1000), anti-GAPDH (Abways, AB0038, 1:5000), anti-GFAP (Cell Signaling Technology, 3670S, 1:1000), anti-GFP (Proteintech, 50430-2-AP, 1:1000), anti-GluA2 ( Article Title: TMEM59 deficiency activates chaperone‐mediated autophagy and ameliorates disease‐like pathologies in tauopathy model mice Article Snippet: Ten to twenty micrograms of protein lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting.. The blots were immunoblotted with primary antibodies, including: anti‐β‐actin (CST, 8457S, 1:2000), anti‐ATF6 (Abcam, ab227830, 1:1000), anti‐BiP (CST, C50B12, 1:1000), anti‐CDK5 (Santa Cruz, sc‐173, 1:1000), anti‐Flag (Proteintech, 20543‐1‐AP, 1:1000), anti‐GAPDH (Abways, AB0038, 1:5000), anti‐GFAP (Cell Signaling Technology, 3670S, 1:1000), anti‐GFP (Proteintech, 50430‐2‐AP, 1:1000), anti‐GluA2 ( Article Title: The regulation and mechanism of the cAMP-PKA pathway on PTSD-like behaviors exacerbated by alcohol exposure Article Snippet: Finally, protein visualization was conducted using the AI-800 imaging system (General Electric Company, United States), and the optical density values of each band were quantitatively analyzed using ImageJ software.and the optical density values of each band were quantitatively analyzed using ImageJ software. ... Primary antibody with the following proteins: anti-p-PKA (Ser133, 5661, 1:1,000, Cell Signaling Technology); anti-PKA (ab32514, 1:1,000, Abcam); anti-p-CREB (Ser133, ab32096, 1:1,000, Abcam); manti-CREB (ab32515, 1:1,000, Abcam); anti-BDNF (ab108319, 1:1,000, Abcam); anti-p-TrkB (ab229908, 1:1,000, Abcam); anti-TrkB (ab187041, 1:2,000, Abcam); anti-PSD95 (20665-1-AP, 1:1,000, Proteintech); anti-Synaptophysin (36406S, 1:1,000, Cell Signaling Technology); anti-NR2A (ab124913, 1:1,000, Abcam); anti-NR2B (ab65783, 1; 1,000, Abcam); anti-GluA1 (13185S, 1:1,000, Cell Signaling Technology); anti-GluA2 ( Article Title: Metaplastic priming enables non-ionotropic NMDA receptor-mediated synaptic depotentiation in the hippocampus Article Snippet: Membranes were briefly stained with 0.1% Ponceau and then incubated in blocking buffer (5% milk in Tris-buffered saline with Tween20 (TBS-T; 20 mM Tris, 150 mM NaCl, 0.1% Tween20)) for 1 hour at room temperature.stained with 0.1% Ponceau and then incubated in blocking buffer (5% milk in Tris-buffered saline with Tween20 (TBS-T; 20 mM Tris, 150 mM NaCl, 0.1% Tween20)) for 1 hour at room temperature. ... The blotted proteins were then probed with moloclonal anti-GluA1 (1:1000, Cell Signaling Technology (CST), Danvers, Massachusetts, United States, cat nr #13185, RRID: AB_2732897), anti-GluA2 ( Article Title: Chronic stress induces Alzheimer’s disease-like pathologies through DNA damage-Chk1-CIP2A signaling Article Snippet: The primary antibodies used in this research were as follows: anti-γH2A.X (ab26350, Abcam; 80312S, Cell Signaling Technology), anti-NeuN (26975-1-AP, Proteintech), anti-GFAP (12389S, Cell Signaling Technology), anti-Iba1 (ab178846, Abcam), anti-Chk1-pS317 (12302S, Cell Signaling Technology), anti-Chk1-pS345 (39233, Gene Tex; A21009, Abclonal), anti-Chk1 (A7653, Abclonal), anti-CIP2A (A12267, Abclonal), anti-Tau-pT231 (R381181, Zen-Bioscience), anti-Tau-pS396 (381213, Zen-Bioscience), anti-Tau-pS404 (11112, SAB), Tau-5 (ab80579, Abcam), anti-APP-pT668 (6986S, Cell Signaling Technology), anti-APP (60,342–1-Ig, Proteintech), anti-PSD95 (36233S, Cell Signaling Technology; 30255-1-AP, Proteintech), anti-Synapsin- I (5297S, Cell Signaling Technology), anti-GluA1 (13185S, Cell Signaling Technology), anti-GluA2 ( Article Title: Chronic Lithium Treatment Alters NMDA and AMPA Receptor Synaptic Availability and Dendritic Spine Organization in the Rat Hippocampus Article Snippet: 9197, RRID: AB_331277), anti- GluA1 (1:1000, Cell Signaling Technology, cod.9197, RRID: AB_331277), anti- GluA1 (1:1000, Cell Signaling Technology, cod.. 13185, RRID: AB_2732897), Staining:Article Title: Astrocytic PYGM attenuates tau pathology by promoting lactate-mediated neuroprotection. Article Snippet: were detected using enhanced chemiluminescence (ECL) and quantified using ImageJ (NIH). .. Primary antibodies used were: anti-α-tubulin (PTM, 5442, 1:10000), anti-β-actin (Abclonal, AC026, 1:10000), anti-Caspase-3 (Cell Signaling Technology, 9662S, 1:1000), anti-Cleaved-Caspase3 (Cell Signaling Technology, 9664S, 1:1000), anti-Cleaved-PARP (Cell Signaling Technology, 9544S, 1:1000), anti-GLAST (Proteintech, 20785-1-AP, 1:1000), anti-GluA1 (Cell Signaling Technology, 13185S, 1:1000), Article Title: Astrocytic PYGM attenuates tau pathology by promoting lactate‐mediated neuroprotection Article Snippet: were detected using enhanced chemiluminescence (ECL) and quantified using ImageJ (NIH). .. Primary antibodies used were: anti‐α‐tubulin (PTM, 5442, 1:10000), anti‐β‐actin (Abclonal, AC026, 1:10000), anti‐Caspase‐3 (Cell Signaling Technology, 9662S, 1:1000), anti‐Cleaved‐Caspase‐3 (Cell Signaling Technology, 9664S, 1:1000), anti‐Cleaved‐PARP (Cell Signaling Technology, 9544S, 1:1000), anti‐GLAST (Proteintech, 20785‐1‐AP, 1:1000), anti‐GluA1 (Cell Signaling Technology, 13185S, 1:1000), Article Title: TMEM59 deficiency activates chaperone-mediated autophagy and ameliorates disease-like pathologies in tauopathy model mice. Article Snippet: Ten to twenty micrograms of protein lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting.. The blots were immunoblotted with primary antibodies, including: anti-β-actin (CST, 8457S, 1:2000), anti-ATF6 (Abcam, ab227830, 1:1000), anti-BiP (CST, C50B12, 1:1000), antiCDK5 (Santa Cruz, sc-173, 1:1000), anti-Flag (Proteintech, 20543- 1-AP, 1:1000), anti-GAPDH (Abways, AB0038, 1:5000), anti-GFAP (Cell Signaling Technology, 3670S, 1:1000), anti-GFP (Proteintech, 50430-2-AP, 1:1000), anti-GluA2 ( Article Title: TMEM59 deficiency activates chaperone‐mediated autophagy and ameliorates disease‐like pathologies in tauopathy model mice Article Snippet: Ten to twenty micrograms of protein lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting.. The blots were immunoblotted with primary antibodies, including: anti‐β‐actin (CST, 8457S, 1:2000), anti‐ATF6 (Abcam, ab227830, 1:1000), anti‐BiP (CST, C50B12, 1:1000), anti‐CDK5 (Santa Cruz, sc‐173, 1:1000), anti‐Flag (Proteintech, 20543‐1‐AP, 1:1000), anti‐GAPDH (Abways, AB0038, 1:5000), anti‐GFAP (Cell Signaling Technology, 3670S, 1:1000), anti‐GFP (Proteintech, 50430‐2‐AP, 1:1000), anti‐GluA2 ( Article Title: The regulation and mechanism of the cAMP-PKA pathway on PTSD-like behaviors exacerbated by alcohol exposure Article Snippet: Finally, protein visualization was conducted using the AI-800 imaging system (General Electric Company, United States), and the optical density values of each band were quantitatively analyzed using ImageJ software.and the optical density values of each band were quantitatively analyzed using ImageJ software. ... Primary antibody with the following proteins: anti-p-PKA (Ser133, 5661, 1:1,000, Cell Signaling Technology); anti-PKA (ab32514, 1:1,000, Abcam); anti-p-CREB (Ser133, ab32096, 1:1,000, Abcam); manti-CREB (ab32515, 1:1,000, Abcam); anti-BDNF (ab108319, 1:1,000, Abcam); anti-p-TrkB (ab229908, 1:1,000, Abcam); anti-TrkB (ab187041, 1:2,000, Abcam); anti-PSD95 (20665-1-AP, 1:1,000, Proteintech); anti-Synaptophysin (36406S, 1:1,000, Cell Signaling Technology); anti-NR2A (ab124913, 1:1,000, Abcam); anti-NR2B (ab65783, 1; 1,000, Abcam); anti-GluA1 (13185S, 1:1,000, Cell Signaling Technology); anti-GluA2 ( Article Title: Metaplastic priming enables non-ionotropic NMDA receptor-mediated synaptic depotentiation in the hippocampus Article Snippet: Membranes were briefly stained with 0.1% Ponceau and then incubated in blocking buffer (5% milk in Tris-buffered saline with Tween20 (TBS-T; 20 mM Tris, 150 mM NaCl, 0.1% Tween20)) for 1 hour at room temperature.stained with 0.1% Ponceau and then incubated in blocking buffer (5% milk in Tris-buffered saline with Tween20 (TBS-T; 20 mM Tris, 150 mM NaCl, 0.1% Tween20)) for 1 hour at room temperature. ... The blotted proteins were then probed with moloclonal anti-GluA1 (1:1000, Cell Signaling Technology (CST), Danvers, Massachusetts, United States, cat nr #13185, RRID: AB_2732897), anti-GluA2 ( Article Title: Chronic stress induces Alzheimer’s disease-like pathologies through DNA damage-Chk1-CIP2A signaling Article Snippet: The primary antibodies used in this research were as follows: anti-γH2A.X (ab26350, Abcam; 80312S, Cell Signaling Technology), anti-NeuN (26975-1-AP, Proteintech), anti-GFAP (12389S, Cell Signaling Technology), anti-Iba1 (ab178846, Abcam), anti-Chk1-pS317 (12302S, Cell Signaling Technology), anti-Chk1-pS345 (39233, Gene Tex; A21009, Abclonal), anti-Chk1 (A7653, Abclonal), anti-CIP2A (A12267, Abclonal), anti-Tau-pT231 (R381181, Zen-Bioscience), anti-Tau-pS396 (381213, Zen-Bioscience), anti-Tau-pS404 (11112, SAB), Tau-5 (ab80579, Abcam), anti-APP-pT668 (6986S, Cell Signaling Technology), anti-APP (60,342–1-Ig, Proteintech), anti-PSD95 (36233S, Cell Signaling Technology; 30255-1-AP, Proteintech), anti-Synapsin- I (5297S, Cell Signaling Technology), anti-GluA1 (13185S, Cell Signaling Technology), anti-GluA2 ( Article Title: Chronic Lithium Treatment Alters NMDA and AMPA Receptor Synaptic Availability and Dendritic Spine Organization in the Rat Hippocampus Article Snippet: 9197, RRID: AB_331277), anti- GluA1 (1:1000, Cell Signaling Technology, cod.9197, RRID: AB_331277), anti- GluA1 (1:1000, Cell Signaling Technology, cod.. 13185, RRID: AB_2732897), Fluorescence:Article Title: Astrocytic PYGM attenuates tau pathology by promoting lactate-mediated neuroprotection. Article Snippet: were detected using enhanced chemiluminescence (ECL) and quantified using ImageJ (NIH). .. Primary antibodies used were: anti-α-tubulin (PTM, 5442, 1:10000), anti-β-actin (Abclonal, AC026, 1:10000), anti-Caspase-3 (Cell Signaling Technology, 9662S, 1:1000), anti-Cleaved-Caspase3 (Cell Signaling Technology, 9664S, 1:1000), anti-Cleaved-PARP (Cell Signaling Technology, 9544S, 1:1000), anti-GLAST (Proteintech, 20785-1-AP, 1:1000), anti-GluA1 (Cell Signaling Technology, 13185S, 1:1000), Article Title: Astrocytic PYGM attenuates tau pathology by promoting lactate‐mediated neuroprotection Article Snippet: were detected using enhanced chemiluminescence (ECL) and quantified using ImageJ (NIH). .. Primary antibodies used were: anti‐α‐tubulin (PTM, 5442, 1:10000), anti‐β‐actin (Abclonal, AC026, 1:10000), anti‐Caspase‐3 (Cell Signaling Technology, 9662S, 1:1000), anti‐Cleaved‐Caspase‐3 (Cell Signaling Technology, 9664S, 1:1000), anti‐Cleaved‐PARP (Cell Signaling Technology, 9544S, 1:1000), anti‐GLAST (Proteintech, 20785‐1‐AP, 1:1000), anti‐GluA1 (Cell Signaling Technology, 13185S, 1:1000), Article Title: TMEM59 deficiency activates chaperone-mediated autophagy and ameliorates disease-like pathologies in tauopathy model mice. Article Snippet: Ten to twenty micrograms of protein lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting.. The blots were immunoblotted with primary antibodies, including: anti-β-actin (CST, 8457S, 1:2000), anti-ATF6 (Abcam, ab227830, 1:1000), anti-BiP (CST, C50B12, 1:1000), antiCDK5 (Santa Cruz, sc-173, 1:1000), anti-Flag (Proteintech, 20543- 1-AP, 1:1000), anti-GAPDH (Abways, AB0038, 1:5000), anti-GFAP (Cell Signaling Technology, 3670S, 1:1000), anti-GFP (Proteintech, 50430-2-AP, 1:1000), anti-GluA2 ( Article Title: TMEM59 deficiency activates chaperone‐mediated autophagy and ameliorates disease‐like pathologies in tauopathy model mice Article Snippet: Ten to twenty micrograms of protein lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting.. The blots were immunoblotted with primary antibodies, including: anti‐β‐actin (CST, 8457S, 1:2000), anti‐ATF6 (Abcam, ab227830, 1:1000), anti‐BiP (CST, C50B12, 1:1000), anti‐CDK5 (Santa Cruz, sc‐173, 1:1000), anti‐Flag (Proteintech, 20543‐1‐AP, 1:1000), anti‐GAPDH (Abways, AB0038, 1:5000), anti‐GFAP (Cell Signaling Technology, 3670S, 1:1000), anti‐GFP (Proteintech, 50430‐2‐AP, 1:1000), anti‐GluA2 ( Article Title: The regulation and mechanism of the cAMP-PKA pathway on PTSD-like behaviors exacerbated by alcohol exposure Article Snippet: Finally, protein visualization was conducted using the AI-800 imaging system (General Electric Company, United States), and the optical density values of each band were quantitatively analyzed using ImageJ software.and the optical density values of each band were quantitatively analyzed using ImageJ software. ... Primary antibody with the following proteins: anti-p-PKA (Ser133, 5661, 1:1,000, Cell Signaling Technology); anti-PKA (ab32514, 1:1,000, Abcam); anti-p-CREB (Ser133, ab32096, 1:1,000, Abcam); manti-CREB (ab32515, 1:1,000, Abcam); anti-BDNF (ab108319, 1:1,000, Abcam); anti-p-TrkB (ab229908, 1:1,000, Abcam); anti-TrkB (ab187041, 1:2,000, Abcam); anti-PSD95 (20665-1-AP, 1:1,000, Proteintech); anti-Synaptophysin (36406S, 1:1,000, Cell Signaling Technology); anti-NR2A (ab124913, 1:1,000, Abcam); anti-NR2B (ab65783, 1; 1,000, Abcam); anti-GluA1 (13185S, 1:1,000, Cell Signaling Technology); anti-GluA2 ( Article Title: Metaplastic priming enables non-ionotropic NMDA receptor-mediated synaptic depotentiation in the hippocampus Article Snippet: Membranes were briefly stained with 0.1% Ponceau and then incubated in blocking buffer (5% milk in Tris-buffered saline with Tween20 (TBS-T; 20 mM Tris, 150 mM NaCl, 0.1% Tween20)) for 1 hour at room temperature.stained with 0.1% Ponceau and then incubated in blocking buffer (5% milk in Tris-buffered saline with Tween20 (TBS-T; 20 mM Tris, 150 mM NaCl, 0.1% Tween20)) for 1 hour at room temperature. ... The blotted proteins were then probed with moloclonal anti-GluA1 (1:1000, Cell Signaling Technology (CST), Danvers, Massachusetts, United States, cat nr #13185, RRID: AB_2732897), anti-GluA2 ( Article Title: Chronic stress induces Alzheimer’s disease-like pathologies through DNA damage-Chk1-CIP2A signaling Article Snippet: The primary antibodies used in this research were as follows: anti-γH2A.X (ab26350, Abcam; 80312S, Cell Signaling Technology), anti-NeuN (26975-1-AP, Proteintech), anti-GFAP (12389S, Cell Signaling Technology), anti-Iba1 (ab178846, Abcam), anti-Chk1-pS317 (12302S, Cell Signaling Technology), anti-Chk1-pS345 (39233, Gene Tex; A21009, Abclonal), anti-Chk1 (A7653, Abclonal), anti-CIP2A (A12267, Abclonal), anti-Tau-pT231 (R381181, Zen-Bioscience), anti-Tau-pS396 (381213, Zen-Bioscience), anti-Tau-pS404 (11112, SAB), Tau-5 (ab80579, Abcam), anti-APP-pT668 (6986S, Cell Signaling Technology), anti-APP (60,342–1-Ig, Proteintech), anti-PSD95 (36233S, Cell Signaling Technology; 30255-1-AP, Proteintech), anti-Synapsin- I (5297S, Cell Signaling Technology), anti-GluA1 (13185S, Cell Signaling Technology), anti-GluA2 ( Article Title: Chronic Lithium Treatment Alters NMDA and AMPA Receptor Synaptic Availability and Dendritic Spine Organization in the Rat Hippocampus Article Snippet: 9197, RRID: AB_331277), anti- GluA1 (1:1000, Cell Signaling Technology, cod.9197, RRID: AB_331277), anti- GluA1 (1:1000, Cell Signaling Technology, cod.. 13185, RRID: AB_2732897), Control:Article Title: Astrocytic PYGM attenuates tau pathology by promoting lactate-mediated neuroprotection. Article Snippet: were detected using enhanced chemiluminescence (ECL) and quantified using ImageJ (NIH). .. Primary antibodies used were: anti-α-tubulin (PTM, 5442, 1:10000), anti-β-actin (Abclonal, AC026, 1:10000), anti-Caspase-3 (Cell Signaling Technology, 9662S, 1:1000), anti-Cleaved-Caspase3 (Cell Signaling Technology, 9664S, 1:1000), anti-Cleaved-PARP (Cell Signaling Technology, 9544S, 1:1000), anti-GLAST (Proteintech, 20785-1-AP, 1:1000), anti-GluA1 (Cell Signaling Technology, 13185S, 1:1000), Article Title: Astrocytic PYGM attenuates tau pathology by promoting lactate‐mediated neuroprotection Article Snippet: were detected using enhanced chemiluminescence (ECL) and quantified using ImageJ (NIH). .. Primary antibodies used were: anti‐α‐tubulin (PTM, 5442, 1:10000), anti‐β‐actin (Abclonal, AC026, 1:10000), anti‐Caspase‐3 (Cell Signaling Technology, 9662S, 1:1000), anti‐Cleaved‐Caspase‐3 (Cell Signaling Technology, 9664S, 1:1000), anti‐Cleaved‐PARP (Cell Signaling Technology, 9544S, 1:1000), anti‐GLAST (Proteintech, 20785‐1‐AP, 1:1000), anti‐GluA1 (Cell Signaling Technology, 13185S, 1:1000), Article Title: TMEM59 deficiency activates chaperone-mediated autophagy and ameliorates disease-like pathologies in tauopathy model mice. Article Snippet: Ten to twenty micrograms of protein lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting.. The blots were immunoblotted with primary antibodies, including: anti-β-actin (CST, 8457S, 1:2000), anti-ATF6 (Abcam, ab227830, 1:1000), anti-BiP (CST, C50B12, 1:1000), antiCDK5 (Santa Cruz, sc-173, 1:1000), anti-Flag (Proteintech, 20543- 1-AP, 1:1000), anti-GAPDH (Abways, AB0038, 1:5000), anti-GFAP (Cell Signaling Technology, 3670S, 1:1000), anti-GFP (Proteintech, 50430-2-AP, 1:1000), anti-GluA2 ( Article Title: TMEM59 deficiency activates chaperone‐mediated autophagy and ameliorates disease‐like pathologies in tauopathy model mice Article Snippet: Ten to twenty micrograms of protein lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting.. The blots were immunoblotted with primary antibodies, including: anti‐β‐actin (CST, 8457S, 1:2000), anti‐ATF6 (Abcam, ab227830, 1:1000), anti‐BiP (CST, C50B12, 1:1000), anti‐CDK5 (Santa Cruz, sc‐173, 1:1000), anti‐Flag (Proteintech, 20543‐1‐AP, 1:1000), anti‐GAPDH (Abways, AB0038, 1:5000), anti‐GFAP (Cell Signaling Technology, 3670S, 1:1000), anti‐GFP (Proteintech, 50430‐2‐AP, 1:1000), anti‐GluA2 ( Article Title: The regulation and mechanism of the cAMP-PKA pathway on PTSD-like behaviors exacerbated by alcohol exposure Article Snippet: Finally, protein visualization was conducted using the AI-800 imaging system (General Electric Company, United States), and the optical density values of each band were quantitatively analyzed using ImageJ software.and the optical density values of each band were quantitatively analyzed using ImageJ software. ... Primary antibody with the following proteins: anti-p-PKA (Ser133, 5661, 1:1,000, Cell Signaling Technology); anti-PKA (ab32514, 1:1,000, Abcam); anti-p-CREB (Ser133, ab32096, 1:1,000, Abcam); manti-CREB (ab32515, 1:1,000, Abcam); anti-BDNF (ab108319, 1:1,000, Abcam); anti-p-TrkB (ab229908, 1:1,000, Abcam); anti-TrkB (ab187041, 1:2,000, Abcam); anti-PSD95 (20665-1-AP, 1:1,000, Proteintech); anti-Synaptophysin (36406S, 1:1,000, Cell Signaling Technology); anti-NR2A (ab124913, 1:1,000, Abcam); anti-NR2B (ab65783, 1; 1,000, Abcam); anti-GluA1 (13185S, 1:1,000, Cell Signaling Technology); anti-GluA2 ( Article Title: Metaplastic priming enables non-ionotropic NMDA receptor-mediated synaptic depotentiation in the hippocampus Article Snippet: Membranes were briefly stained with 0.1% Ponceau and then incubated in blocking buffer (5% milk in Tris-buffered saline with Tween20 (TBS-T; 20 mM Tris, 150 mM NaCl, 0.1% Tween20)) for 1 hour at room temperature.stained with 0.1% Ponceau and then incubated in blocking buffer (5% milk in Tris-buffered saline with Tween20 (TBS-T; 20 mM Tris, 150 mM NaCl, 0.1% Tween20)) for 1 hour at room temperature. ... The blotted proteins were then probed with moloclonal anti-GluA1 (1:1000, Cell Signaling Technology (CST), Danvers, Massachusetts, United States, cat nr #13185, RRID: AB_2732897), anti-GluA2 ( Article Title: Chronic stress induces Alzheimer’s disease-like pathologies through DNA damage-Chk1-CIP2A signaling Article Snippet: The primary antibodies used in this research were as follows: anti-γH2A.X (ab26350, Abcam; 80312S, Cell Signaling Technology), anti-NeuN (26975-1-AP, Proteintech), anti-GFAP (12389S, Cell Signaling Technology), anti-Iba1 (ab178846, Abcam), anti-Chk1-pS317 (12302S, Cell Signaling Technology), anti-Chk1-pS345 (39233, Gene Tex; A21009, Abclonal), anti-Chk1 (A7653, Abclonal), anti-CIP2A (A12267, Abclonal), anti-Tau-pT231 (R381181, Zen-Bioscience), anti-Tau-pS396 (381213, Zen-Bioscience), anti-Tau-pS404 (11112, SAB), Tau-5 (ab80579, Abcam), anti-APP-pT668 (6986S, Cell Signaling Technology), anti-APP (60,342–1-Ig, Proteintech), anti-PSD95 (36233S, Cell Signaling Technology; 30255-1-AP, Proteintech), anti-Synapsin- I (5297S, Cell Signaling Technology), anti-GluA1 (13185S, Cell Signaling Technology), anti-GluA2 ( Article Title: Chronic Lithium Treatment Alters NMDA and AMPA Receptor Synaptic Availability and Dendritic Spine Organization in the Rat Hippocampus Article Snippet: 9197, RRID: AB_331277), anti- GluA1 (1:1000, Cell Signaling Technology, cod.9197, RRID: AB_331277), anti- GluA1 (1:1000, Cell Signaling Technology, cod.. 13185, RRID: AB_2732897), Expressing:Article Title: Astrocytic PYGM attenuates tau pathology by promoting lactate-mediated neuroprotection. Article Snippet: were detected using enhanced chemiluminescence (ECL) and quantified using ImageJ (NIH). .. Primary antibodies used were: anti-α-tubulin (PTM, 5442, 1:10000), anti-β-actin (Abclonal, AC026, 1:10000), anti-Caspase-3 (Cell Signaling Technology, 9662S, 1:1000), anti-Cleaved-Caspase3 (Cell Signaling Technology, 9664S, 1:1000), anti-Cleaved-PARP (Cell Signaling Technology, 9544S, 1:1000), anti-GLAST (Proteintech, 20785-1-AP, 1:1000), anti-GluA1 (Cell Signaling Technology, 13185S, 1:1000), Article Title: Astrocytic PYGM attenuates tau pathology by promoting lactate‐mediated neuroprotection Article Snippet: were detected using enhanced chemiluminescence (ECL) and quantified using ImageJ (NIH). .. Primary antibodies used were: anti‐α‐tubulin (PTM, 5442, 1:10000), anti‐β‐actin (Abclonal, AC026, 1:10000), anti‐Caspase‐3 (Cell Signaling Technology, 9662S, 1:1000), anti‐Cleaved‐Caspase‐3 (Cell Signaling Technology, 9664S, 1:1000), anti‐Cleaved‐PARP (Cell Signaling Technology, 9544S, 1:1000), anti‐GLAST (Proteintech, 20785‐1‐AP, 1:1000), anti‐GluA1 (Cell Signaling Technology, 13185S, 1:1000), Article Title: TMEM59 deficiency activates chaperone-mediated autophagy and ameliorates disease-like pathologies in tauopathy model mice. Article Snippet: Ten to twenty micrograms of protein lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting.. The blots were immunoblotted with primary antibodies, including: anti-β-actin (CST, 8457S, 1:2000), anti-ATF6 (Abcam, ab227830, 1:1000), anti-BiP (CST, C50B12, 1:1000), antiCDK5 (Santa Cruz, sc-173, 1:1000), anti-Flag (Proteintech, 20543- 1-AP, 1:1000), anti-GAPDH (Abways, AB0038, 1:5000), anti-GFAP (Cell Signaling Technology, 3670S, 1:1000), anti-GFP (Proteintech, 50430-2-AP, 1:1000), anti-GluA2 ( Article Title: TMEM59 deficiency activates chaperone‐mediated autophagy and ameliorates disease‐like pathologies in tauopathy model mice Article Snippet: Ten to twenty micrograms of protein lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting.. The blots were immunoblotted with primary antibodies, including: anti‐β‐actin (CST, 8457S, 1:2000), anti‐ATF6 (Abcam, ab227830, 1:1000), anti‐BiP (CST, C50B12, 1:1000), anti‐CDK5 (Santa Cruz, sc‐173, 1:1000), anti‐Flag (Proteintech, 20543‐1‐AP, 1:1000), anti‐GAPDH (Abways, AB0038, 1:5000), anti‐GFAP (Cell Signaling Technology, 3670S, 1:1000), anti‐GFP (Proteintech, 50430‐2‐AP, 1:1000), anti‐GluA2 ( Article Title: The regulation and mechanism of the cAMP-PKA pathway on PTSD-like behaviors exacerbated by alcohol exposure Article Snippet: Finally, protein visualization was conducted using the AI-800 imaging system (General Electric Company, United States), and the optical density values of each band were quantitatively analyzed using ImageJ software.and the optical density values of each band were quantitatively analyzed using ImageJ software. ... Primary antibody with the following proteins: anti-p-PKA (Ser133, 5661, 1:1,000, Cell Signaling Technology); anti-PKA (ab32514, 1:1,000, Abcam); anti-p-CREB (Ser133, ab32096, 1:1,000, Abcam); manti-CREB (ab32515, 1:1,000, Abcam); anti-BDNF (ab108319, 1:1,000, Abcam); anti-p-TrkB (ab229908, 1:1,000, Abcam); anti-TrkB (ab187041, 1:2,000, Abcam); anti-PSD95 (20665-1-AP, 1:1,000, Proteintech); anti-Synaptophysin (36406S, 1:1,000, Cell Signaling Technology); anti-NR2A (ab124913, 1:1,000, Abcam); anti-NR2B (ab65783, 1; 1,000, Abcam); anti-GluA1 (13185S, 1:1,000, Cell Signaling Technology); anti-GluA2 ( Article Title: Metaplastic priming enables non-ionotropic NMDA receptor-mediated synaptic depotentiation in the hippocampus Article Snippet: Membranes were briefly stained with 0.1% Ponceau and then incubated in blocking buffer (5% milk in Tris-buffered saline with Tween20 (TBS-T; 20 mM Tris, 150 mM NaCl, 0.1% Tween20)) for 1 hour at room temperature.stained with 0.1% Ponceau and then incubated in blocking buffer (5% milk in Tris-buffered saline with Tween20 (TBS-T; 20 mM Tris, 150 mM NaCl, 0.1% Tween20)) for 1 hour at room temperature. ... The blotted proteins were then probed with moloclonal anti-GluA1 (1:1000, Cell Signaling Technology (CST), Danvers, Massachusetts, United States, cat nr #13185, RRID: AB_2732897), anti-GluA2 ( Article Title: Chronic stress induces Alzheimer’s disease-like pathologies through DNA damage-Chk1-CIP2A signaling Article Snippet: The primary antibodies used in this research were as follows: anti-γH2A.X (ab26350, Abcam; 80312S, Cell Signaling Technology), anti-NeuN (26975-1-AP, Proteintech), anti-GFAP (12389S, Cell Signaling Technology), anti-Iba1 (ab178846, Abcam), anti-Chk1-pS317 (12302S, Cell Signaling Technology), anti-Chk1-pS345 (39233, Gene Tex; A21009, Abclonal), anti-Chk1 (A7653, Abclonal), anti-CIP2A (A12267, Abclonal), anti-Tau-pT231 (R381181, Zen-Bioscience), anti-Tau-pS396 (381213, Zen-Bioscience), anti-Tau-pS404 (11112, SAB), Tau-5 (ab80579, Abcam), anti-APP-pT668 (6986S, Cell Signaling Technology), anti-APP (60,342–1-Ig, Proteintech), anti-PSD95 (36233S, Cell Signaling Technology; 30255-1-AP, Proteintech), anti-Synapsin- I (5297S, Cell Signaling Technology), anti-GluA1 (13185S, Cell Signaling Technology), anti-GluA2 ( Article Title: Chronic Lithium Treatment Alters NMDA and AMPA Receptor Synaptic Availability and Dendritic Spine Organization in the Rat Hippocampus Article Snippet: 9197, RRID: AB_331277), anti- GluA1 (1:1000, Cell Signaling Technology, cod.9197, RRID: AB_331277), anti- GluA1 (1:1000, Cell Signaling Technology, cod.. 13185, RRID: AB_2732897), |